What if AI could design a brand-new protein, from scratch, to tackle heart disease? That’s no longer science fiction. In a breakthrough collaboration, 310.ai and Arctoris have validated the first de novo protein from 310’s MP4 platform that binds ATP – a critical step towards restoring energy in failing hearts.
Targeting the Root Cause of Heart Failure
Heart failure affects over 64 million people globally. Its core issue? A breakdown in energy metabolism – specifically, ATP depletion in cardiomyocytes. Despite its prevalence and clear pathophysiology, existing treatments often miss this underlying cause.
310.ai’s MP4 platform designed a novel enzyme optimized for ATP regeneration. Unlike natural adenylate kinases, this protein, while ~70% similar to natural adenylate kinases, features AI-designed structural enhancements that boost stability, binding, and catalysis beyond evolutionary constraints.
From Molecule Design to Bench Validation
Arctoris brought the design to life using its Ulysses® automation platform, expressing and purifying the protein across multiple host systems in large quantities and outstanding quality. Downstream, a suite of biophysical assays confirmed the protein folding, purity, monodispersity and, crucially, high ATP-binding activity, validating MP4’s ability to generate functional enzymes de novo.
What’s next for this AI-designed breakthrough? Arctoris is now validating the protein, precisely quantifying its adenylate kinase activity using advanced mechanistic enzymology. Stay tuned for what we uncover!
A Glimpse into the Future: MP4 and Beyond
MP4 is a state-of-the-art generative model trained on over 3.2 billion datapoints, capable of turning text prompts into functioning proteins. The upcoming MP5 will go further, designing non-protein therapeutics, integrating pharma-specific constraints, and enabling complex, multi-modal drugs.
Why This Matters
If you’re designing novel biologics or building ML models, Arctoris provides the infrastructure to make your ideas real. Our work with 310.ai shows how computational design and high-quality experimental validation can come together, rapidly, reliably, and at scale.
Need robust data to train your models or validate new therapeutic modalities? Ulysses®, our automated platform, delivers fast, reproducible insights to support your discovery pipeline.
Acknowledgments
Arctoris is proud to partner with 310.ai, advancing AI-driven protein design. We also thank AMD Instinct for providing the computational power behind MP4’s training.
Explore the full benchmarks and white papers at 310.ai.